Fig. 4: Non-enzymatic triazole assembly.
From: Nitric oxide as a source for bacterial triazole biosynthesis
a LC-MS analysis of the reaction mixtures of 5, 6, or 7 with NaNO2 or DEA NONOate. The EICs for 4 (m/z 363, [M−H]−), 3 (m/z 283, [M−H]−), and 1 (m/z 151, [M−H]−) are presented. Compound 5, 6, or 7 (0.5 mM) are incubated with DEA NONOate (1 mM) or sodium nitrite (1 mM) at 30 °C for 1 h before LC-MS analysis. Authentic 1, 3, 4 were used as analytical standards. b Change of 6 (75 μM, black line) absorption spectra upon the addition of NONOate (150 μM) in 50 mM phosphate buffer (pH 7.4) at 25 °C. The red trace was collected 1 h after the addition of NONOate. The absorbance at 310 nm was monitored with a 1.5-min time interval (as shown in the insert figure). c Time-course study of the reaction of 6 (75 μM) with NONOate (150 μM) or nitrite (150 μM). Red arrow indicates the time point when NONOate or nitrite was added. Values are means of three independent experiments ± SD. Source data are provided as a Source Data file. d LC-MS analysis of the reaction mixtures of 6 (75 μM) and NONOate (1 mM) prepared anaerobically and aerobically. The EICs for 4 (m/z 363, [M−H]−) are presented. Three independent tests were taken.
