Fig. 6: The active-site loop is a determinant structural difference between enzymatically active and inactive Grx.

a, b Selected \(k_{{\mathrm{cat}}}^{{\mathrm{app}}}\) and \(K_{\mathrm{m}}^{{\mathrm{app}}}\) values of ScGrx7 wild-type enzyme, ScGrx7^WP, ScGrx7^loop, ScGrx7^WP+loop, and HsGrx5^loop. c Calculated catalytic efficiencies from panels (a) and (b). d Reciprocal Dalziel coefficients, which probably reflect the rate constants of the oxidative and reductive half-reaction with GSSCys and GSH, respectively. e Summary of the altered kinetic parameters. Source data are provided in the Supplementary Information: original plots and kinetic parameters for panels a–c are shown in Supplementary Figs. 13 and 15 and Supplementary Tables 8 and 9. Statistical analyses and P values for the \(k_{{\mathrm{cat}}}^{{\mathrm{app}}}\) and \(K_{\mathrm{m}}^{{\mathrm{app}}}\) values from panels a and b are listed in Supplementary Table 11. Error bars are the calculated standard error from the curve fits in SigmaPlot 13. Reciprocal Dalziel coefficients for panel d were obtained from Supplementary Figs. 14 and 16 and are listed together with the true k_cat values in Supplementary Table 7.