Fig. 4: Western blot validation of proteome data.
From: The translational landscape of ground state pluripotency
Graphs showing the RNA, RFP, and protein expression levels of selected candidate genes based on RNA-seq, RFP-seq, and LC-MS proteomic analysis. Western blot analysis confirms the proteome data for the selected proteins in 2iL, SL, and EPI states and during 2iL–Sl transition. Candidate proteins included 2iL-specific proteins, BHMT, NQO1, and KRAS; SL-specific protein, S100A6; SL and 2iL-specific protein, ESRRB and a general pluripotency protein (SL, 2iL, and EPI), OCT4. Note that Kras is regulated at the post-translational level.
