Fig. 6: Experimental growth factor stimulations in PAECs confirms priming.

a Log2 fold-change of the gene expression (as measured by RT-qPCR) and the H3K27ac signal (as measured by ChIP-Seq) of the regulatory element(s) linked to the gene are shown as scatter plot. Each point is a regulatory element-gene pair. The fold-changes are calculated between PAH patients and controls in steady state for H3K27ac and after a stimulation with endothelial-specific growth factors (serotonin—yellow triangles, vascular endothelial growth factor (VEGF)—red crosses, and transforming growth factor β (TGFβ)—blue squares) for the RNA. Pearson correlation coefficients (R) and associated p-values are given in the plots. (qPCR experiments: n = 4 patients, four controls). b Western blot analyses are shown for a selection of genes that showed a differential response to any of the stimulations on the RNA level. GAPDH levels are shown as a reference. Source data are provided in Source Data File. c ChIP-qPCR analysis for enhancers predicted to prime response in PAH patients. H3K27ac signal at selected enhancers (Enh; orange, brown and pink box for NOS3, YAP1, and TGFBR2), was measured after TGFβ stimulation in patient and controls. All of the enhancers showed a trend in the predicted direction and some of them statistically significant (n = 3 patients, three controls). Error bars indicate standard deviation across three replicates.