Fig. 5: GEP1 interacts with GCα in gametocytes. | Nature Communications

Fig. 5: GEP1 interacts with GCα in gametocytes.

From: An intracellular membrane protein GEP1 regulates xanthurenic acid induced gametogenesis of malaria parasites

Fig. 5: GEP1 interacts with GCα in gametocytes.The alt text for this image may have been generated using AI.

a Top 10 GEP1 interacting proteins in the gametocytes of the 6HA::gep1 parasite detected by immunoprecipitation and mass spectrometry (MS), including guanylyl cyclase α (GCα) with 15 peptides detected. b MS2 spectrum of a representative peptide of the GCα protein. c Co-immunoprecipitation of Myc::GEP1 and GCα::HA proteins in gametocytes of the double tagged parasite 4Myc::gep1/gcα::6HA (DTS1). IP-Myc, anti-Myc antibody was used. d Co-immunoprecipitation of HA::GEP1 and GCα::Myc proteins in gametocytes of the double tagged parasite 6HA::gep1/gcα:: 4Myc (DTS2). IP-Myc, anti-Myc antibody was used. e Two-colored IFA of GEP1 and GCα proteins in the DTS1 gametocytes using anti-HA (GCα) and anti-Myc (GEP1) antibodies (left panel). Cross sections (white dash line) of the cells show the co-localization of GEP1 and GCα (right panel). Scale bar = 5 μm. f Pearson coefficient analysis for GEP1 and GCα co-localization shown in e, data are shown as mean ± SD from n = 10 cells measured. Experiments in c, d, and e were repeated three times independently with similar results.

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