Fig. 1: Neuronal eIF4E overexpression elevates anxiety without altering social interaction.
From: Elevated protein synthesis in microglia causes autism-like synaptic and behavioral aberrations
a Syn1-Cre;R26Eif4e/Eif4e (NN4E) mice overexpress eIF4E in neurons, while R26Eif4e/Eif4e mice serve as controls (Ctrl). b Levels of eIF4E in hippocampal extracts prepared from control and NN4E mice. The eIF4E immunoblot reveals both endogenous eIF4E (lower band) and overexpressed eIF4E-Myc (upper band). Alpha tubulin was used as a loading control. n = 3 per genotype. **p = 0.0015 by two-sided t-test. c Protein synthesis in cultured hippocampal neurons as revealed by puromycin incorporation. n = 3 mice per genotype. **p = 0.0023 and *p = 0.015 by two-sided t-test. d Time spent in the central zone during the first 5 min in open field tests. Male: 15 control mice and 13 NN4E mice; Female: 15 control mice and 13 NN4E mice. **p = 0.0076, *p = 0.0218 by two-sided t-test. e Percentage of marbles buried. n = 15 control mice and 13 NN4E mice. *p = 0.0247 by two-sided t-test. f, g Sociability of male (f) and female (g) NN4E mice as revealed in three-chamber sociability tests. Male: 17 control mice and 14 NN4E mice; Female: 14 control mice and 13 NN4E mice. Two-way analysis of variance (ANOVA) with Fisher’s LSD post-hoc test: *p < 0.05, **p < 0.01, ***p < 0.001, and n.s. not significant. All data are shown as mean ± s.e.m. Source data are provided as a Source Data file.
