Fig. 3: Iba1 immunohistochemistry reveals altered microglial density and morphology in male MG^4E mice.

a–c Increased microglial density and size in the mPFC of male MG^4E mice. n = 7 control mice and 6 MG^4E mice. Ten microglia from each mouse were randomly selected for measurement of cell size (cross section area). *p = 0.0342 and ***p < 0.001 by two-sided t-test. Scale bar, 100 μm. d–f Comparable microglial density and size in the mPFC between female control and MG^4E mice. n = 6 control mice and 5 MG^4E mice. Five to ten microglia from each mouse were randomly selected for measurement of cell size (cross section area). n.s. not significant by two-sided t-test. Scale bar, 100 μm. g Three-dimension reconstruction of microglia in the mPFC of 2-week-old male mice. n = 7 mice per group. Three to five microglia from each mouse were reconstructed for determination of cell size (volume) and number of processes. **p = 0.0037 and ***p = 0.0005 by two-sided t-test. Scale bar, 20 μm. h–m Microglia density and size in the mPFC of 6-week-old male (h–j) and female (k–m) MG^4E mice. Ten microglia in each brain region of each mouse were randomly selected for measurement of cell size (cross section area). Male, n = 6 mice per genotype; female, n = 5 control mice and 6 MG^4E mice. Two-sided t-test: ***p < 0.001 and n.s. not significant. Scale bar, 100 µm. n Three-dimension reconstruction of microglia in the mPFC of 6-week-old male mice. Four microglia from each mouse were reconstructed. Six control mice and five MG^4E mice. *p = 0.0385 by two-sided t-test. Scale bar, 20 µm. All data are shown as mean ± s.e.m. Source data are provided as a Source Data file.