Fig. 5: Quantitative proteomics and real-time PCR analyses.
From: Mild proteasomal stress improves photosynthetic performance in Arabidopsis chloroplasts
a, b Volcano plot of the results from quantitative proteome profiling with the different genotypic lines. Data on the x-axis represent the Log2-ratio between the absolute abundances of proteins identified from the sp1 (left plot) and rpn8a (right plot) single mutants against wildtype (a) and the Log2-ratio of absolute abundances between the sp1 ppi2 (left plot) and the rpn8a ppi2 double mutants against ppi2 (b). The y-axis represents the Log10 of the p-value from a two-sided T-test. Significant changes are indicated by the gray box (ratio > 1.5 times different, p-value < 0.05, T-test, two-sided). All data are based on three biological replicates that were measured in three technical replicates each. c Quantitative real-time PCR results obtained with the transcripts of selected genes for photosynthetic proteins. Significant differences from three biological replicates are highlighted by a star (T-test, two-sided, p-value < 0.05), error-bars represent SD.
