Fig. 5: RAMS11 induces liver metastasis via hemisplenectomy mouse model.
From: Long non-coding RNA RAMS11 promotes metastatic colorectal cancer progression
a Representative mice showing no liver metastasis in RAMS11 CRISPR KO cell-injected mice by BLI. b RAMS11 CRISPR KO cell-injected mice show a significant decrease in liver metastasis by day 21. c Day 21 ex vivo mouse livers show decreased metastasis in RAMS11 CRISPR KO cell-injected mice by BLI. Wild-type cell-injected mice had d increased liver weights and e liver metastasis compared with CRISPR KO cell-injected mice. f Hematoxylin and eosin stain of livers showing metastasis (M) and levels of Ki67 stain. Three independent tissues were stained per group. White bar = 10 µM, black bar = 100 µM. Data shown as mean ± SEM and analyzed by two-tailed paired t-test, with WT n = 18, CRISPR1 n = 11, CRISPR2 n = 11 per group, experiment was repeated three times. *p < 0.05, **p < 0.005, #p < 0.0005. Source data are provided as a Source Data File.
