Fig. 6: Synthetic hybrids as a tool to study genome instability.

a Box plots of the number of chromosomal aberrations inferred for ancestor and evolved synthetic hybrids (Fig. 2b, Supplementary Fig. 3) (n = 1 sequenced strain). Synthetic hybrids generated from each independent scheme are represented with different shapes. Purple and brown color points represent whether six-species hybrids were ancestor or evolved, respectively. Median values are represented by a horizontal line inside the box, and the upper and lower whiskers represent the highest and lowest values of the 1.5 × IQR (interquartile range), respectively. b For each colony isolated from the population sample of the evolved synthetic hybrid yHRWh88, the genome contribution of each Saccharomyces species is stacked, and the percentage of retention is indicated inside the bar plot. The percentage of each species’ contribution are colored according to the legend. c The number of chromosomes were inferred from sppIDer plots and corrected based on flow cytometry. The chromosome content was colored according to the species donor. The numbers of chromosomes for each species are colored according to the heatmap legend. Recombinant chromosomes are colored in gray. Asterisks indicate chromosomes that were retained in a particular colony but were not observed in the evolved yHWRh88 population sample, highlighting the instability of these hybrids. S. cerevisiae chromosome IV, where the xylose utilization genes were inserted, is indicated by the black square. Source data are provided in the Source Data file and at http://bit.ly/2v1rq1T.