Fig. 2: TAGAP is required for Dectin-2/3 and mincle ligands-induced signaling activation.

a BMDMs from heterozygous control mice or TAGAP-deficient mice were stimulated with α-Mannan (100 μg/ml) for 3 and 6 h, followed by real-time PCR analysis of indicated genes. b BMDMs from heterozygous control mice or TAGAP-deficient mice were stimulated with α-Mannan (100 μg/ml) for indicated times, followed by western blot analysis of indicated protein expression. c Control- gRNA or TAGAP-gRNA knocked down THP-1 cells were stimulated with α-mannan (100 μg/ml) for indicated times, followed by western blot analysis of indicated proteins. d BMDMs from heterozygous control mice or TAGAP-deficient mice were stimulated with TDB (50 μg/well) for 3 and 6 h, followed by real-time PCR analysis of indicated genes. e BMDMs from heterozygous control mice or TAGAP-deficient mice were stimulated with TDB (50 μg/well) for indicated times, followed by western blot analysis of indicated protein expression. *P < 0.05; **P < 0.01; ***P < 0.001 based on two-sided unpaired t test a, d. All error bars represent SEM of technical replicates. Data are representative of three independent experiments.