Fig. 5: TAGAP is important for T helper cell polarization in mice.

a Cells of lymph nodes and spleens from heterozygous control or TAGAP-deficient mice were analyzed by flow cytometry by gating on CD4⁺, and analyzed by IL-17A or IFN-γ-producing cells. Right panel indicates quantitative result, and the top right panel represents data from spleen, and the bottom right data represent data from lymph nodes (n = 8). b Heterozygous control or TAGAP-deficient mice were immunized subcutaneously with 200 μg MOG_35–55 and 400 μg Mycobacteria tuberculosis H37RA in 200 μL of complete Freund’s adjuvant (Difco). Lymph nodes were collected 10 days later and single-cell suspensions were prepared. Cells were cultured with MOG_35–55 (20 μg/mL) for another 3 days, followed by flow cytometry analysis of indicated cell populations. Right panel were quantitative results (n = 4). c Heterozygous control or TAGAP-deficient mice were immunized as in b, except for immunization with 200 μg MOG_35–55 plus CFA and 400 μg heat-killed C. albicans (sc-5314). Right panel were quantitative results (n = 4). d Heterozygous control or TAGAP-deficient mice were immunized as in b, except for immunization with 200 μg MOG_35–55 plus CFA only (n = 4). ns: P = 0.5226 and P = 0.288. e Naive CD4⁺ T cells were isolated from spleens of heterozygous control or TAGAP-deficient mice, and polarized in vitro for 3 days in the Th17 or Th1 cell polarization conditions. Cells were analyzed by flow cytometry by gating on CD4⁺, and analyzed by IL-17A or IFN-γ-producing cells. Right panel were quantitative results (n = 10). ns: P = 0.1848 and P = 0.9952. f Wild-type mice were injected intravenously with 2 × 10⁵ live C. albicans (sc-5314) in 100 μL PBS. Ten days later, CD4⁺ T cells were isolated from spleen, and co-cultured with DCs isolated from heterozygous control or TAGAP-deficient mice for another 3 days together with indicated numbers of heat-killed C. albicans. Cells were analyzed by flow cytometry by gating on CD4⁺, and analyzed by IL-17A or IFN-γ-producing cells (n = 6). Right panel were qualified results. *P < 0.05; **P < 0.01; ***P < 0.001 based on two-sided unpaired t test a–f. All error bars represent SEM of technical replicates. Data are representative of two independent experiments.