Fig. 4: Characterizing the nucleic acid-binding modes of Sox2-HMG.
a Equilibrium competition binding assays measuring the anisotropy of the labeled nucleic acid as a function of unlabeled competitor. Binding curves presented as the average of n = 3 technical replicates with the error bars reflecting their s.d. b Determination of complex stoichiometry by EMSA. Migration of the radiolabeled nucleic acid species as a function of [Sox2-HMG]:[Ligand]. Bands are denoted as F (free), B1 (bound species 1), and B2 (bound species 2). Quantification provided in Supplementary Fig. 5. c Analysis of the salt dependence of the Sox2-HMG/nucleic acid interactions. The colored bars show the measured binding affinities as the average of the n = 4 with the standard error reported (black shapes). The calculated slope of each linear regression is provided. d The calculated values of the electrostatic (ΔGel) and non-electrostatic (ΔGnel) components to binding the indicated nucleic acid ligand under the standard reaction conditions.
