Fig. 7: Sox2 interacts with RNA in mESC.
a Schematic of fRIP and UV-RIP-Seq experimental flow. b, c Examples of enriched genes (Pmepa1 and Brd2) identified by both formaldehyde (“CH2O”) and UV-based RIP-seq. Each panel describes normalized coverage of sequencing reads (RPM: reads per million reads). d Correlation of enrichment between fRIP and UV-RIP. A dot represents a gene and each axis describes the fold change of IP relative to input in log scale. A color indicates statistical significance of enrichment (FDR-adjusted p-value<0.05): blue significant only by formaldehyde, orange: significant only by UV, red: significant by both formaldehyde and UV. e Overlap of genes enriched in both fRIP and UV-RIP. Data for both fRIP (n = 4) and UV-RIP (n = 4) are obtained from eight total distinct cell culture plates.
