Fig. 3: Removing podoplanin removes the signal for platelet CLEC-2 signalling and enhances liver healing after CCl4 or APAP induced liver injury.

WT (Cre negative) or podoplanin deficient (Vav1-iCre⁺ pdpn^fl/fl) mice were injected with IP CCl₄ (1 mg/kg) and sacrificed at 48 h or IP APAP (350 mg/kg) and sacrificed at 24 h. a Serum ALT level is shown from mice treated with either APAP (acetaminophen) or CCl₄, the first and third pairs of columns compare WT mice that were pretreated with either a podoplanin function blocking antibody (white columns) or the corresponding isotype matched control antibody (black columns) (n = 6–8). The second and fourth column pairs compare genetically deficient podoplanin mice (Vav1-iCre⁺PDPN^fl/fl-white columns) and WT controls-black columns (n = 4–8). b Representative hematoxylin and eosin stained sections from the time points mentioned above are shown, images acquired at ×10 magnification using a Leica CTR6000 microscope with QCapture software, images representative of at least four different mice in each group (scale bar—100 µm). Black arrows highlight areas of hepatic necrosis. Differences assessed using either unpaired Students t-test or a Mann–Whitney test *P < 0.05, **P < 0.01, ***P < 0.001. Each data point is representative of an independent animal and data is presented as mean ± SEM.