Fig. 3: Multi-register and sense-antisense recording.
From: DNA punch cards for storing data on native DNA sequences via enzymatic nicking
a Five orthogonal registers used instead of one single register. Each vertical section represents one register in genome dictated reading order, and each row shows the read lengths retrieved after sequencing analysis. Read lengths are recorded on the left and sequencing depths on the right axis. b Nicking both the sense and antisense strands. Here, the binary format is switched to ternary format, i.e., for each nicking site, a nick on the sense strand denotes 1, a nick on the antisense denotes 2, and no nick denotes 0. The coverage (left) and size distribution (right) plots verify that the register can be nicked on ten prescribed sites on both strands, six of which are located on the sense strand and four on the antisense strands, corresponding to the 10-bit string of data: 1212121211. The sense-antisense nicking approach may also be used for parallel recording on multiple registers. Source data are available in the Source Data file.
