Fig. 5: Pattern of RNA used by PRC2 on EcoRI-digested DNA templates.
From: RNA-DNA strand exchange by the Drosophila Polycomb complex PRC2
a–c Schematic of DNA and RNA templates for the vg (a), bxd (b) and dia (c) PREs. DNA template were linearized at one of the indicated sites. d, e. Schematic of RNA and DNA templates. RNA transcripts were produced from linear templates that end four bases before the EcoRI site so that the sequence indicated is not present in the RNAs. f, g Schematics of the four possible hybrids formed using the 2 RNAs and the sense (f) or anti-sense (g) DNA, indicating which two are observed. h–j show representative gels for each data set. Experiments were repeated three times, and all four sets for each PRE were always run in the same experiment. [PRC2] is 25–400 nM.
