Fig. 10: Alloxan inhibits Gfat1-induced hypertrophic growth.
From: Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling
a Inhibition of O-GlcNAcylation with alloxan led to a decrease in cardiomyocyte growth from Gfat1 overexpression. Adenovirus-mediated overexpression of Gfat1 increased cardiomyocyte size, which was reduced by alloxan treatment in NRVMs. Scale: 20 μM. N = 80 cells for Ad-GFP/veh; n = 88 cells for Ad-Gfat1/veh; n = 135 cells for Ad-GFP/alloxan; n = 118 cells for Ad-Gfat1/alloxan. At least three independent experiments were conducted with two to three samples/group/experiment. b Suppression of O-GlcNAcylation caused a decrease in the expression of molecular markers of cardiomyocyte hypertrophy in NRVMs. Western blotting was conducted. In contrast to Rcan1.4, Rcan1.1 expression remained unaltered. N = 8–29. c The mTOR signaling was significantly inhibited by alloxan treatment. N = 3–11. Data are shown as mean ± SEM. Significance was calculated by two-way ANOVA, followed by Tukey’s test. *p = 0.003; ***p < 0.001. Source data are provided as a Source Data file.
