Fig. 8: Both mTORC1 and mTORC2 contribute to Gfat1-mediated cardiomyocyte growth.
From: Chronic activation of hexosamine biosynthesis in the heart triggers pathological cardiac remodeling
a Knockdown of either Raptor or Rictor reduced Gfat1-mediated cardiomyocyte growth. NRVMs were transfected by siRNAs against Raptor or Rictor along with controls. Adenovirus expressing GFP or Gfat1 was then used to infect the cells. After immunofluorescent staining, cardiomyocyte size was quantified (right). Scale: 20 μM. N = 55–153/cells for each group. At least three independent experiments were conducted with 2–3 samples/group/experiment. b Protein expression of βMHC and Rcan1 was determined by Western blotting. Quantification at the right showed a significant decrease in expression by knockdown of either Raptor or Rictor, indicating both mTORC1 and mTORC2 contribute to Gfat1-mediated cardiomyocyte growth. N = 5–8. Data are shown as mean ± SEM. Significance was calculated by two-way ANOVA, followed by Tukey’s test. ***p < 0.001. Source data are provided as a Source Data file.
