Fig. 2: Quantitative comparison of MP with nsEM for anaphase-promoting complex/cyclosome (APC/C) purification and crosslinking.
From: Quantifying the heterogeneity of macromolecular machines by mass photometry
a Structural cartoon including highlights of all 14 subunits. b Details of the three-step purification protocol and fractions analyzed. c SDS-PAGE gels of all fractions highlighted in b. d Corresponding mass distributions of purification step fractions and APC/CCDH1-UBE2C and APC/CCDH1-UBE2S traps indicated as UBE2C and UBE2S, respectively. e Comparison of assembled fractions obtained by MP (circle) and nsEM (triangle). For the evaluation of mole fractions, we did not consider species below 400 kDa to avoid errors from buffer background for the trap samples. Error bars are calculated as the standard deviation of mole fractions from different repeats. f 2D class averages of the two most populated classes obtained by each step are shown along with simulated projections of either APC/C or APC/CPlatform. Source data are provided as a Source Data file.
