Table 1 DiscoverMP analysis parameters for all protein complexes. The values for threshold 2 (0.25) and median filter kernel (15) remained constant for all protein samples.
From: Quantifying the heterogeneity of macromolecular machines by mass photometry
Protein | Number of binned frames, nf | Threshold 1 (T1) |
|---|---|---|
Respiratory complex I | 5 | 0.5 |
Trimeric cohesin | 5 | 0.8 |
NPC | 5 | 0.8 |
APC/C—affinity chromatography | 3 | 2 |
APC/C—ion-exchange chromatography | 5 | 3 |
APC/C—size-exclusion chromatography | 3 | 2 |
APC/C—crosslinked | 3 | 2 |
Proteasome | 5 | 3.5 |
