Fig. 8: Purified Cthrc1+ pathologic fibroblasts showed high migration and invasion capacity. | Nature Communications

Fig. 8: Purified Cthrc1+ pathologic fibroblasts showed high migration and invasion capacity.

From: Collagen-producing lung cell atlas identifies multiple subsets with distinct localization and relevance to fibrosis

Fig. 8: Purified Cthrc1+ pathologic fibroblasts showed high migration and invasion capacity.The alternative text for this image may have been generated using AI.

a Gating strategy for purifying collagen-producing populations from bleomycin-treated lungs. b qPCR analysis of purified cells from bleomycin-treated lungs (day 10). n = 4 mice. c Representative images from gap migration assay with cells purified from bleomycin-treated lungs (day 10). Broken lines show initial cell-free zones. Col-GFP is shown in green. Scale bars, 500 μm. d Quantification of migration assay. n = 4 mice. ***p < 0.001, two-way analysis of variance followed by the Tukey–Kramer post-test. e The number of GFP+ cells from the host lungs, which received purified cells from bleomycin-treated lungs, were analyzed. n = 5 (adventitial) or 6 (cluster 8 and alveolar) mice. **p < 0.01, ***p < 0.001, one-way analysis of variance followed by the Tukey–Kramer post-test. f Whole lung imaging of host lungs. Col-GFP (donor cells) is shown in green. Autofluorescence in RFP channel is shown in white to visualize host lung cells. Images were maximum projection of z-stack images. Scale bars, 1 mm. b–f Data are representative from two experiments. b, d, e Data are means ± SEM. Source data are provided as a Source Data file.

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