Fig. 5: Activation of callosal pathway alone from either eye is not sufficient to drive spiking.

a Schematic of experimental design showing inactivation of ipsilateral LGN (muscimol injection) and reversible inactivation of vc pathway. b Cartoon depicting pathways deactivated during muscimol injection (upper) for ipsilateral stimulation and for experiments with muscimol injection and vc pathway inactivation (lower). c Cartoon depicting pathways deactivated during muscimol injection (upper) for contralateral stimulation and for experiments with muscimol injection and vc pathway inactivation (lower). d Comparison of spiking response for maximum response angle during ipsilateral (left, 8 neurons, ipsilaterally responsive, 2 animals) and contralateral (right, 8 neurons, contralaterally responsive, 2 animals) only stimulation. Control (dark blue, no muscimol), vc pathway inactivation (magenta), LGN inactivation (purple), and LGN inactivation with vc pathway inactivation (yellow). Spontaneous activity rates (black) shown for each neuron. Ipsilateral responses not different to spontaneous activity on vc pathway silencing indicated by orange lines. e Left panel, example field of view with labeled neuropil ROI (shaded area); right panel, neuropil responses evoked by stimulation of ipsilateral (left) or contralateral (right) eye recorded during silencing of ipsilateral LGN without (upper) and with VCPN-inactivation (lower). Individual (gray) and average (black) responses. Shuffled neuropil responses (white). Stimulus onset (black arrow head) and offset (gray arrow head).