Fig. 6: The intracomplex phosphorylation connects G1-CDK priming and S-CDK activity. | Nature Communications

Fig. 6: The intracomplex phosphorylation connects G1-CDK priming and S-CDK activity.

From: A processive phosphorylation circuit with multiple kinase inputs and mutually diversional routes controls G1/S decision

Fig. 6

a, b Plots showing the distributions of Sic1 destruction timing values of individual cells in case of unperturbed cell cycle for strains expressing mutated variants of either full-length Sic1-EGFP in a or Sic1ΔC-EGFP in b. In case of Sic1ΔC-EGFP strains, endogenous Sic1 was expressed in the background. The median value along with 95% confidence intervals are denoted by black lines on the plot. The number of individual cells (X) observed over a number of individual colonies (Y) is given in form n = X(Y). The values for mother and daughter cells separately and the additional information are presented in Supplementary Table 1 and in the Source data file for Fig. 6. c A table of measured Sic1 destruction timing median values for the indicated strains. d A viability assay of strains with sic1::sic1(9SP) and sic1::sic1(8SP-T173) genomic replacements and conditional GAL1-CLB5 overexpression. e A viability assay of strains with sic1-deletion and sic1::sic1(T173A) genomic replacement in cln1,2-deletion background and conditional GAL1-CLN3 overexpression. The experiments for panels ‘d’ and ‘e’ were performed twice with similar result. f, g Schemes showing the difference in diversional termination mechanism for the borderline pheromone arrest (f), and for G1/S transition (g). Source data are provided as a Source Data file.

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