Table 4 Utilization of carbohydrates in TT or TS-Coculture Broths by various T. thermosaccharolticum strains with heterologous expression of identified enzymes.

From: Development of a thermophilic coculture for corn fiber conversion to ethanol

α-Xylp _1211

α-l-Galp _687

α-l-Galp _697

α-Araf _1120

α-Araf _996

β-Xylp _1710

% Carbohydrate utilization TT-Coculture Broth

Plasmid

Blank (Empty vector)

8 ± 5%

pLL1270

     

32 ± 2%

pLL1271

  

  

34 ± 3%

pLL1272

   

 

37 ± 2%

pLL1274

    

38 ± 2%

pLL1273

 

 

44 ± 6%

pLL1277

 

 

48 ± 6%

pLL1275

 

 

49 ± 7%

pLL1276

  1. Growth experiments were done on 2 different days with replicates n ≥ 4, with two different Coculture Broths from 20 g/L corn fiber fermentations: TT-Coculture Broth with 4.3 g/L or TS-Coculture Broth with 4.0 g/L total carbohydrates. Fresh media components were not added other than 5 g/L MOPS.