Fig. 3: MLL-AF9 leukemia is delayed in Mbnl1 knockout mice.
From: MBNL1 regulates essential alternative RNA splicing patterns in MLL-rearranged leukemia
a Scheme of mouse leukemia model. Wild type (WT) or knockout (KO) lineage negative bone marrow cells were transduced with an MLL-AF9 GFP-expressing retrovirus, followed by serial replating in semisolid medium to select for MLL-AF9 cells. Selected cells were then transplanted into irradiated primary recipient mice (n = 10 per group). b Graph represents percent of leukemia-free survival of primary recipient mice. ***p = 0.0003 by two-sided Log-rank (Mantel-Cox) test. c Distressed primary recipient mice 12 weeks after transplantation were sacrificed and splenocytes were harvested and plated for CFU assay. After 7 days in semi-solid culture spleen cells were injected into secondary irradiated recipients (n = 5 mice per group). Graph depicts percent of leukemia-free survival. **p = 0.0088 by two-sided Log-rank (Mantel-Cox) test.
