Fig. 5: MBNL1-regulated alternative splicing is active in leukemic cells.
From: MBNL1 regulates essential alternative RNA splicing patterns in MLL-rearranged leukemia
a RT-PCR analysis of regions of select MBNL1 regulated genes (INF2, ATP2A1, and CD47) across a panel of MLL-rearranged and wild-type leukemia cells. b RT-PCR analysis of INF2, ATP2A1, and CD47 transcripts demonstrating change in splicing pattern in MOLM13 cells after MBNL1 knockdown. Band percentages (relative to each individual lane) are noted. c Alternative splicing patterns of MBNL1 target genes are shown after 48 h of Compound 1 treatment at the indicated dosages. Relative proportion of each AS feature indicated in decimal adjacent to corresponding band. All gels are representative images of two biological replicates.
