Fig. 5: Adaptation of CheY-F6 to function with F7 systems in stages 3–5. | Nature Communications

Fig. 5: Adaptation of CheY-F6 to function with F7 systems in stages 3–5.

From: Repurposing a chemosensory macromolecular machine

Fig. 5

a Comparison of the sequence logos of the CheY proteins from the F6 system and CheY-F6-like present in genomes with 3–5 F7 systems. The amino-acids are color coded according to their chemical properties: neutral (purple), polar (green), positive charge (blue), negative charge (red), and hydrophobic (black). The dots mark conserved positions within each group that are similar between stages 3–5 but in the group of CheY-F6. b The alpha carbons (yellow spheres) of these positions are mapped in the structure of CheY (red) bound to the P1 and P2 domains of CheA (blue). From the ten positions that matched our criteria, only two of them were not located at an interface with CheA (M45K and T56S). Three residues are facing P2 (R92K, Q94N, and V103A) and five are facing P1 (K22R, R26K, D27E, D37E, and G62N). Note that the numbers refer to the coordinates of the E. coli CheY.

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