Fig. 2: Optically evoked responses and ChR2-expressing CDt axon terminals in SNr and GPe. | Nature Communications

Fig. 2: Optically evoked responses and ChR2-expressing CDt axon terminals in SNr and GPe.

From: Optogenetic manipulation of a value-coding pathway from the primate caudate tail facilitates saccadic gaze shift

Fig. 2

a Recording from CDt neurons during optical stimulation in CDt. MSNs in CDt receive inhibitory inputs from FSIs and excitatory inputs from TANs, and have projections to cdlSNr or cvGPe. The ChR2 vector was non-selectively transfected in all groups of CDt neurons. b A representative MSN showing an excitatory response to optical stimulation (20 ms duration) (P = 0.0062, Wilcoxon singed-rank test, two-sided). Raster plots show the activity aligned to the stimulation onset. PSTHs show the average response of the neuron to the stimulation across trials. Light-blue shade indicates the stimulation period (0–20 ms). An inset shows the MSN’s spike waveform. **indicates P < 0.01. c The average activity of four out of seven MSNs showing significant excitatory responses to the optical stimulation (P < 0.05, Wilcoxon singed-rank test, two-sided). d ChR2-positive neurons in CDt and PUTt of monkey SH. Green signal indicates enhanced EYFP signal. Scale bar: 1 mm. Histological results of monkey ZB replicated the ChR2-positive neurons in CDt and PUTt. e Left: ChR2-positive axon terminals in cdlSNr of monkey SH. Scale bar: 1 mm. Right: enlarged image of the axons in cdlSNr (the area shown by white box in left). Scale bar: 50 μm. The results of monkey ZB replicated the ChR2-positive axon terminals in cdlSNr. f Left: ChR2-positive axon terminals in cvGPe of monkey SH. Right: enlarged image of the axons in cvGPe (the area shown by white box in left). Scale bar: 50 μm. The results of monkey ZB replicated the ChR2-positive axon terminals in cvGPe.

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