Fig. 4: Absence or blockade of E-selectin stimulates quiescent AML blasts to cycle, while HSPC remain dormant. | Nature Communications

Fig. 4: Absence or blockade of E-selectin stimulates quiescent AML blasts to cycle, while HSPC remain dormant.

From: Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance

Fig. 4

a Flow cytometry dot plots showing gating strategy for AML blasts (GFP+ Lin−KIT+CD11b+) and HSPC (GFP− Lin− KIT+ Sca1+, LSK) within the same leukemic host from mice administered 24 h BrdU. The cell cycle phases were defined as G0 (Ki-67−, BrdU−), G1 (Ki-67+, BrdU−) and S-G2-M (Ki-67+, BrdU+). b Histograms show percentage of bone marrow LSK HSPC and AML blasts in quiescence (phase G0, left panel) and proliferative (BrdU+, right panel). Each dot represents data from an individual mouse. Shown are mean ± S.D., n = 4,5,4 mice/group. Statistical analysis performed by one-way ANOVA multiple comparison Bonferroni correction. Source data are provided as a Source Data file.

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