Fig. 2: Brg1 reexpression rescues rKO mice.
a Survival of rKO1 mice. rKO1 mice were either Brg1F/ΔR; FoxP3YFP-Cre; R26CAG-FlpoER/CAG-FlpoER males or Brg1F/ΔR; FoxP3YFP-Cre/YFP-Cre; R26CAG-FlpoER/CAG-FlpoER females. Littermate controls were of the same genotypes, except that they carried either Brg1F/+ or Brg1ΔR/+ and were thus heterozygous for Brg1. For convenience, these controls were labeled “WT” throughout the paper. The rKO1 mice treated with the full dose of TAM lived significantly longer than rKO1 (p = 0.01, log-rank test). b Representative image of rKO1 mice before and after TAM treatment (full dose). c Body weight gain of rKO1 mice following TAM treatment (full dose). * and **P values ≤ 0.05 and 0.001, respectively. d, e Abundance of effector/memory-like (E/M) and naive CD4 cells in peripheral blood. TAM (full dose) was given to 3-week-old rKO mice (Day 0). Blue and red asterisks denote statistical significance when Naive and E/M CD4 frequencies, respectively, were compared between rKO1 and WT mice. *P values ≤ 0.05 and 0.001. f Low-dose TAM regimen fully rescued rKO2 mice (P = 0.0007, Student’s t test). For comparison, the survival curve for the rKO1 mice (a) is also displayed (red line). g rKO2 mice were nearly as runted as rKO1. h Body weight (left) and E/M CD4 cells (right) of the five TAM-treated rKO2 mice in f. The mean values (±SEM) of WT littermates are also plotted (dotted lines). As the rKO mice (bearing five to six alleles) were quite rare, the five mice were born and hence analyzed at different times, except for the last two time points when the mice from different litters were analyzed together (the mouse with 8% reversal analyzed on Days 151 and 251).
