Fig. 6: PZ rejuvenates HSCs in naturally aged mice.

a A representative flow cytometric gating strategy of lineage negative (Lin⁻) cells, hematopoietic progenitor cells (HPC or Lin⁻sca1^–c-kit⁺cells), LSK cells (Lin^–sca1⁺c-kit⁺cells), and Scal1^–c-Kit^low cells, HSC (Lin^–sca1⁺c-kit⁺CD48^–CD150⁺ cells), granulocyte–monocyte progenitors (GMP or Lin^–sca1^–c-kit⁺CD34⁺CD16/32⁺), megakaryocyte–erythrocyte progenitors (MEP or Lin^–sca1^–c-kit⁺CD34^–CD16/32^–), common myeloid progenitors (CMP or Lin^–sca1^–c-kit⁺CD34⁺CD16/32^–), and common lymphoid progenitors (CLP or Lin^–Sca1^–c-Kit^low CD127⁺CD135⁺) in the bone marrow (BM) cells from control untreated young mice (Young) and naturally aged mice treated with vehicle (VEH), ABT263 (ABT) or PZ as shown in Fig. 3b. b Representative flow cytometric analyses of HPC, LSK cells, Sca1^–c-Kit^low cells, HSC, CMP, MEP, CMP, and CLP in BM cells from the mice shown in Fig. 3b. Data are percentages of indicated cell counts in the cell populations labeled on the top of each panel. The number in each gate represents percentage of the gated cells in the cell population marked on the top of each panel. c Total BM cell counts in the mice shown in Fig. 3b. The percentages of BM HPC (d), LSK (e), HSC (f), CMP (g), MEP (h), GMP (i), and CLP (j) cells in total BM cells from the mice shown in Fig. 3b. k. Percentage of SA-β-gal positive senescent LSK cells in LSK cells from the mice shown in Fig. 3b. The data presented in c–k are mean ± SEM (n = 8, 8, 6, and 7 mice for the Young, VEH, ABT263, and PZ groups). a, b, c p < 0.05 vs. Young, Aged + VEH, and Aged + ABT, respectively. l. The percentage of individually sorted HSCs produced more than 10,000 cells in cultures. The data presented are mean ± SEM (n = 4 independent assays for Young, Aged + ABT, and Aged + PZ groups and n = 5 independent assays for Aged + VEH group). a, b p < 0.05 vs. Young, Aged + VEH, respectively. Data in c to l were analyzed by one-way ANOVA with Tukey’s post-hoc tests. Source data are provided as a Source Data file. The exact P values are provided in the Source Data file.