Fig. 2: Binding of PARP1 to DNA strand break-mimicking oligonucleotides.
From: Real-time monitoring of PARP1-dependent PARylation by ATR-FTIR spectroscopy
a Representative time-dependent spectra of PARP1 binding to immobilised DNAblunt. b Evaluation of time-dependent binding of PARP1 to different types of DNA strand breaks. ‘0 min’ refers to the first time point measured. Signal intensities of amide I bands (1645 cm−1) at 20 min were set to 100%. Binding kinetics were calculated via a mono-exponential fit function. c Comparison of the amount of PARP1 bound to different types of DNA stand breaks after 20 min of co-incubation. Amide I bands (1645 cm−1) were normalised to the amount of immobilised DNA (1220 cm−1). b, c Data from same experiments. Means ± SEM of n = 14 (DNAblunt) and n = 3 (DNA3’P, DNA5’P, DNAnick) independent experiments, respectively. d Secondary structure analysis. Difference spectra of amide I bands of PARP1 bound to DNAblunt and DNA3’P, DNA5’P or DNAnick were calculated. Average curves and SD (grey) of nine difference spectra are plotted (n = 3 independent experiments, respectively). Source data are provided as a Source Data file.
