Fig. 2: 15N relaxation rates of YY-β1AR and TS-β1AR.
a, b Residue-specific 15N apparent transverse relaxation rates R2,app of 15N-valine YY-β1AR and TS-β1AR derived from line shape fitting of the 1H-15N TROSY spectra in the apo form and orthosteric binary complexes recorded at 304 K and 21 T (YY-β1AR) or 19 T (TS-β1AR). Due to limited stability, the apo form of YY-β1AR was recorded at 294 K. Data are color-coded according to the ligand. The dashed lines represent theoretical TROSY 15N R2 rates for a rotational correlation time τc of 38 ns. c, d Residue-specific 15N spin-echo transverse (R2,spin-echo) and longitudinal (R1) rates of alprenolol-bound 15N-valine TS-β1AR recorded at 304 K and 21 T (red) or 14 T (blue). Dashed lines represent theoretical R2 and R1 rates. The assignment of V60 is tentative and marked by an asterisk. Numerical data for a–d are provided in Supplementary Data 3. Error values were derived from Monte Carlo fitting as indicated in Methods. e Average 15N relaxation rates obtained from 1D experiments on 2H (~60%)/15N-labeled, alprenolol-bound TS-β1AR (Supplementary Fig. 3), molecular weight by MALS, and rotational correlation times τc derived from NMR and MALS.
