Table 2 Knockout cell lines display reduced HCP content during a purification process.

From: Multiplex secretome engineering enhances recombinant protein production and purity

  

HCCF

Protein A

CIEX

AIEX

Total

WT

mAb (µg)

38,470

33,135

26,371

17,088

 
 

Purification yield (%)

 

86.1

79.6

64.8

44.4

 

HCP (ppm)

1,795,217

7243

158

45

 
 

LRV

 

2.4

1.7

0.5

4.6

6xKO

mAb (µg)

43,480

41,891

31,904

24,104

 
 

Purification yield (%)

 

96.3

76.2

75.6

55.4

 

HCP (ppm)

472,873

1596

74

18

 
 

LRV

 

2.5

1.3

0.6

4.4

11xKO

mAb (µg)

12,180

10,881

7377

4308

 
 

Purification yield (%)

 

89.3

67.8

58.4

35.4

 

HCP (ppm)

1,638,998

551

47

3

 
 

LRV

 

3.5

1.1

1.2

5.8

  1. The HCCF, protein A elution pool, CIEX elution pool, and AIEX flow through pool of WT Rit, 6xKO Rit, and 11xKO Rit were subjected to mAb and HCP titer measurements. These values were used to calculate yield, HCP LRV, and HCP content in ppm. Mean of technical duplicates is shown.
  2. CIEX cation exchange, AIEX anion exchange, LRV log reduction value, ppm parts per million.
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