Fig. 3: MALT-1 functions in RMG interneurons.
From: MALT-1 mediates IL-17 neural signaling to regulate C. elegans behavior, immunity and longevity
a A MALT-1::mCherry translational fusion, expressed from its endogenous promoter (4 kb), is expressed in RMG interneurons. RMG is recognized by its characteristic shape, location, and using a flp-5p::gfp reporter. Similar results were obtained in 3 experiments. Scale bars: 20 μm. b Expressing malt-1 cDNA from either the flp-5 promoter (RMG, ASG, PVT, I4, M4, and pharyngeal muscle), or the gcy-32 promoter (URX, AQR and PQR) rescues the aggregation defect of malt-1 mutants. N = 4 assays. Data are presented as mean values +/− SEM. **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey’s post hoc HSD. c The O2-response defect of malt-1 mutants is partially rescued by expressing malt-1 cDNA from the flp-5 promoter (RMG, ASG, PVT, I4, M4, and pharyngeal muscle), or the gcy-32 promoter (URX, AQR and PQR), and almost completely rescued when malt-1 is expressed from both promoters simultaneously. Lines indicate average speed and shaded regions indicate SEM. n = 55 animals (npr-1), n = 85 animals (npr-1; malt-1), n = 58 animals (npr-1; malt-1; gcy-32p::malt-1), n = 66 animals (npr-1; malt-1; flp-5p::malt-1), n = 46 animals (npr-1; malt-1; gcy-32p::malt-1, flp-5p::malt-1). Plots show average speed (line) and SEM (shaded regions). *P < 0.05, **P < 0.01, ***P < 0.001, two-sided Mann-Whitney U test.
