Fig. 1: Viability and distribution of mutations in nitrogen-starved cultures.
From: Nitrogen starvation reveals the mitotic potential of mutants in the S/MAPK pathways
a The ability to form colonies (CFUs) was monitored on 17 independent cultures for 3 months by plating aliquots of the cultures on rich medium at various time points (black curve). The mean is represented and the gray area delineates the limits of the standard deviations. The red (wild-type reference strain 972 h-) and blue (our wild-type progenitor PB1623) curves were made in parallel on a culture that was split into three on day 1 of quiescence. The CFUs were determined in triplicate at days 21, 61, 73, 79, and 90. The three phases are highlighted. Source data are provided as a Source Data file. b Analysis of the DNA content of PB1623 cells determined by FACS at 1, 15, 21, 30, 40, and 50 days of starvation. c Distribution of the number of mutations per genome after 3 months of starvation in the 36 randomly picked (R) colonies and in the 12 colonies selected for their sensitivity to various agents. d Schematic representation of the pathways in which the nine genes analyzed in this study operate.
