Fig. 3: Clonal evolutionary history and parsimonious metastatic map of Patient_8.

a Oval plots showing the subclonal structure of tumor samples from Patient_8. Each row represents a sample. Ovals in the same color represent the same mutation clusters and are denoted by numbers. The area of each oval is proportional to its CCF value. Subclones are shown with solid borders. Subclonal structures are illustrated by the nested ovals to the left. The mutation cluster clonal in all lesions (cluster 1) is the trunk cluster, representing the most recent ancestor clone (MRCA). Other mutation clusters (clusters 2–7) shared by two or more lesions are defined as branch clusters, representing branch subclones. White asterisks denote monoclonal metastases. b Clonal evolutionary tree inferred from the subclonal structure. Lengths of lines are proportional to the number of substitutions in each cluster. Selected aberrations are labeled accordingly. LOH, loss of heterozygosity. c Clonal evolutionary history. The dashed box shows the predicted historical states of the primary tumor. Horizontal arrows denote acquisition of new subclones and tumor progression. Vertical fishbone-like arrows denote metastases, which are labeled with involved clone/subclones. Each colored line corresponds to a clone/subclone. d Parsimonious metastatic map based on the clonal evolutionary history. Each metastasis is colored by the smallest involved subclone.