Fig. 2: CSA interacts with the newly identified C-terminal CIM of CSB.
From: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II
a Outline of the chromatin-tethering approach in U2OS 2-6-3 cells. b A schematic representation of CSB and its deletion mutants. c Recruitment of CSA-GFP to the LacO array upon tethering of the indicated mCherry-LacR fusion proteins (scale bar = 5 µm). See Supplementary Figs. 3 and 4 for a full overview of all tested mutants. d Quantification of CSA-GFP and mCherry-LacR-CSB co-localization at the LacO array. Each symbol represents the mean of an independent experiment (n = 2 for all except LacR-NLS and LacR-CSBWT which is n = 8, >50 cells collected per experiment). e Sequence alignment of CSB orthologues. See Supplementary Fig. 5 for additional alignments. Source data are provided as a Source Data file.
