Fig. 6: UVSSA is the key protein that recruits TFIIH.
From: The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II
a A schematic representation of UVSSA WT and deletion mutants. The CSA-interacting region (CIR) and TFIIH-interacting region (TIR) are indicated. b Recruitment of CSA-GFP and TFIIH (p89) to the LacO array upon tethering of the indicated mCherry-LacR fusion proteins (scale bar = 5 µm). c Quantification of CSA-GFP and endogenous TFIIH (p89) co-localization at the LacO array. Each symbol represents the mean of an independent experiment (n = 2, >50 cells collected per experiment). d Western blot analysis of U2OS (FRT) and UVSSA-KO cells complemented with GFP-UVSSAWT, GFP-UVSSAΔCIR, and GFP-UVSSAΔTIR (n = 2). e Co-IP of GFP-UVSSAWT, GFP-UVSSAΔCIR, and GFP-UVSSAΔTIR. f Endogenous RNAPIIo Co-IP in GFP-UVSSAWT, GFP-UVSSAΔCIR, and GFP-UVSSAΔTIR cell lines. At least two independent replicates of each IP experiment were performed obtaining similar results. See also Supplementary Fig. 7b, c for additional Co-IP data. Source data are provided as a Source Data file.
