Fig. 4: CUBIC-HV is applicable to various antibodies and dyes.
a Results of whole-brain staining and LSFM imaging with various antibodies (yellow) and nuclear stains (SYTOX-G or BOBO-1, blue). The data were reconstructed with Imaris software. The staining, imaging and image processing conditions are summarized in Supplementary Data 2. Voxel size 8.3 × 8.3 × 9 μm3. L: left, R: right. Scale: 2 mm. b Nine sets of the whole-brain imaging data in a (stained with anti-phospho-Nf, calbindin D28K, PV, Sst, ChAT, Dbh, Th, Tph2, or Copeptin antibodies, respectively, with nuclear stains) were registered and aligned to the whole-brain images obtained with anti-NeuN antibody staining and nuclear staining (structural standard). All the transformed data were merged as a pseudo-multitarget whole-brain antibody staining image with Imaris software. The precise registration results are indicated by the brain regions merged across different datasets, such as the paraventricular nucleus (PVN) and the supraoptic nucleus (SON) (NeuN+/copeptin+), the locus coeruleus (LC) in the pons (Dbh+/Th+) and the Purkinje cell layer in the cerebellum (calbindin D28K+/PV+). L left, R right. Scale: 2 mm (whole-brain), 1 mm (reconstituted coronal sections), 0.5 mm (inset).
