Fig. 7: CUBIC-HV allows whole-organ cellular circuit analysis.
a Experimental schematic of whole-brain rabies virus (RV) tracing with cell-type immunolabeling. b Whole-brain image of the RV-injected Gad2-Cre adult mouse brain reconstituted with Imaris software. TVA-mCherry and RV-GFP were injected into left M1 region of the cerebral cortex. Voxel size 8.3 × 8.3 × 9 μm3. Scale: 2 mm. c Anti-Sst antibody staining channel was merged with the image in b. Scale: 2 mm. d, e Enlarged horizontal section images at the indicated positions in c, showing the RV-labeled neurons in the local and ipsilateral corticocortical circuits. Scale: 0.5 mm. f Reconstituted sagittal (y–z) section at the position indicated in d. Scale: 0.5 mm. g-j Cropped and enlarged images at the positions indicated in f. Starter neurons (green and magenta) and input neurons (green) with or without an Sst signal could be identified with single-cell resolution. Scale: 0.1 mm.
