Fig. 8: CUBIC-HV allows whole-organ cellular function analysis.
a Experimental schematic of whole-brain c-Fos antibody staining in different pharmacological conditions. b Whole-brain images of c-Fos-immunolabeled adult mouse brains with or without administration of NMDA receptor antagonist MK-801. The images were reconstituted with Imaris software. Voxel size 6.5 × 6.5 × 7 μm3. The white box indicates the position of the reconstituted sagittal image in d. Scale: 2 mm. c Resulting images following application of a convolution filter to the images in b. Scale: 2 mm. d Reconstituted sagittal images of brains in b, showing uniform labeling of c-Fos-expressing cells. The c-Fos-expressing cells of the dentate gyrus (DG) region were more obvious in the MK-801 (−) sample (arrowheads in insets). Scale: 1 mm (whole sagittal image) and 0.5 mm (insets).
