Fig. 9: CUBIC-HV enables organ-level comparison of rodent and primate brains.
a Experimental schema of the whole-organ comparison analysis of rodent and primate brains. b Comparison of an adult marmoset brain hemisphere with the whole adult mouse brain (only the right hemisphere is depicted), costained with SYTOX-G, GFAP (A594), and α-SMA (A647) antibodies. Note that the orientations were different between samples. Images were reconstituted with Imaris software. Voxel sizes 16.4 × 16.4 × 16 μm3 (for marmoset brain) and 8.3 × 8.3 × 9 μm3 (for mouse brain). MCA middle cerebral artery, PCA posterior cerebral artery, BA basilar artery. Scale: 2 mm. c Reconstituted coronal sections of hemisphere images in b, showing the distributions of corresponding vessel structures and GFAP-positive cells. Note that the different orientations of the sections (z–x and x–z for the marmoset and mouse samples, respectively) could be compared because of the almost isotropic voxel resolution of the z-stack images. ACA anterior cerebral artery, Str striatum, Hp hippocampus. Scale: 2 mm. d Magnified images at the positions indicated in c, showing the similar distributions of vessel-associating GFAP-positive cells in the hippocampus. Voxel size in both images 8.3 × 8.3 × 9 μm3. Scale: 0.5 mm. e Flowchart of staining-based whole-organ imaging and image analysis on CUBIC-HistoVIsion.
