Table 4 Percent of motile wildtype and mutant actin–Tpm filaments determined from in vitro analysis.

Filament type	±Tpm	[Myosin] (µg ml⁻¹)	Filaments moving (%)	n
F-Act88F^WT	−	12.5	31.02 ± 1.64	22
F-Act88F^M305L	−	12.5	32.57 ± 2.34	21
F-Act88F^WT	+	12.5	14.96 ± 1.49	20
F-Act88F^M305L	+	12.5	21.17 ± 2.11*	21
F-Act88F^WT	−	50	68.11 ± 1.51	16
F-Act88F^M305L	−	50	68.00 ± 2.30	20
F-Act88F^WT	+	50	56.43 ± 1.04	21
F-Act88F^M305L	+	50	67.81 ± 1.37^#	21
F-Act88F^WT	−	100	64.09 ± 1.64	19
F-Act88F^M305L	−	100	67.89 ± 1.61	22
F-Act88F^WT	+	100	62.87 ± 1.83	20
F-Act88F^M305L	+	100	71.16 ± 1.78**	22

Data are presented as mean ± SEM. *P≤0.05, **P≤0.01, ^#P≤0.0001 Significantly different from F-Act88F^WT+Tpm. Significant differences between filament types ± Tpm at each myosin concentration were assessed using a two-way ANOVA with Bonferroni post-tests. Source data are provided as a Source Data file.

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