Fig. 5: Differential drug sensitivity of cells associated with two trajectories. | Nature Communications

Fig. 5: Differential drug sensitivity of cells associated with two trajectories.

From: Multi-omic single-cell snapshots reveal multiple independent trajectories to drug tolerance in a melanoma cell line

Fig. 5

a MITF-GFP reporter cell line was sorted for MITF-High and MITF-Low subpopulations before drugging. The sorted cells were then treated with BRAFi + NFκBi combination for 5 days and then collected for cell number counting. Relative cell survival of sorted MITF-High and MITF-Low cells after undergoing BRAFi + NFκBi combination therapy for 5 days were plotted. Survival data were normalized to the MITF-High sample. Each experiment is the result of n = 4 biologically independent samples per group. b MITF-GFP reporter cell line was sorted for MITF-High and MITF-Low subpopulations before drugging. The sorted cells were then treated with BRAFi + PKM2i combination for 5 days and then collected for cell number counting. Relative cell survival of sorted MITF-High and MITF-Low cells after undergoing BRAFi + PKM2i combination therapy for 5 days were plotted. Survival data were normalized to the MITF-Low sample. Each experiment is the result of n = 4 biologically independent samples per group. c MITF-knockdown cells and control cells were treated with BRAFi + NFκBi combination for 5 days and then collected for cell number counting. Relative cell survival of sorted control and MITF-sh cells after undergoing BRAFi + NFΚBi combination therapy for 5 days were plotted. Survival data were normalized to the control sample. Each experiment is the result of n = 5 biologically independent samples per group. d MITF-knockdown cells and control cells were treated with BRAFi + PKM2i combination for 5 days and then collected for cell number counting. Relative cell survival of sorted control and MITF-sh cells after undergoing BRAFi + PKM2i combination therapy for 5 days were plotted. Survival data were normalized to the MITF-sh sample. Each experiment is the result of n = 4 biologically independent samples per group. For boxplots in a–d, data are median with first and third quartiles (box), and top and bottom quartiles (whiskers) indicated. e M397 cell treated with BRAFi, BRAFi + NFΚBi, BRAFi + PKM2i, and BRAFi + NFκBi + PKM2i for 5 days were collected for cell number counting. Relative cell survival of cells after undergoing BRAFi, BRAFi + NFκBi, BRAFi + PKM2i, or BRAFi + PKM2i + NFκBi therapy for 5 days were plotted. Survival data were normalized to cells undergoing BRAFi monotherapy treatment. Each experiment is the result of n = 4 biologically independent samples per group. The P-value was determined by a two-tailed unpaired Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001. Data are presented as mean values ± SEM. Source data are provided as a Source Data file.

Back to article page