Fig. 2: Pbrm1 knockout reduced Ifngr2 expression in Renca cells.

a BRG1 and STAT1 binding to Cxcl9 and Cxcl10 promoter in Renca cells. b BRG1 and SP1 binding to Ifngr2 promoter. Chromatin immunoprecipitation (ChIP) with BRG1, STAT1 or SP1 antibody as indicated in figures was performed using SimpleChIP® Plus Enzymatic Chromatin IP Kit. Isotype IgG was used as negative control. Immunoprecipitated DNA was amplified and quantified by real-time PCR. Protein relative occupancy on promoter was expressed as a percent of the total input chromatin. Con KO or Pbrm1 KO Renca cells were treated with 1 ng/ml IFNγ for 2 h for IFNγ-induced STAT1 binding to Cxcl9 or Cxcl10 promoter. c IFNγ receptor subunits, Ifngr1 and Ifngr2, mRNA expression detected by real-time PCR. Gapdh was used as internal control. d IFNγ receptor subunits, IFNGR1 and IFNGR2, protein expression was detected by western blot. β-actin was used an internal control. e IFNGR2 membrane expression was detected by flow cytometry. Unpaired t-test was performed with GraphPad Prism 7.03. *P < 0.05 and **P < 0.001, compared with control knockout cells. Data in the bar graphs represent mean ± S.D. Source data are provided as a Source Data file.