Table 1 Key resources table.
From: Estrogen receptor-α expressing neurons in the ventrolateral VMH regulate glucose balance
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
Goat anti-WGA antibody | VectorLabs | AS-2024 |
Biotinylated donkey anti-goat secondary antibody | Jackson ImmunoResearch | 705-065-003 |
Chemicals, peptides, and recombinant proteins | ||
Angiotensin II | Sigma | A9525 |
CaCCinh-A01 | Tocris | 4877 |
Tolbutamide | Sigma | T0891 |
TTX | Tocris | 1078 |
4-AP | Tocris | 0940 |
Insulin | Lily | HI-210 |
Glucose | Sigma | G8270 |
2-DG | Sigma | D6134 |
D-AP5 | Tocris | 0106 |
Bicuculline | Tocris | 0131 |
Experimental models: organisms/strains | ||
Mouse: Esr1-Cre | Jackson Laboratory | 017911 |
Mouse: ERα-ZsGreen | Saito, et al.17 | N/A |
Mouse: Rosa26-tdTOMATO | Jackson Laboratory | 007909 |
Recombinant DNA | ||
CAV2-Cre | Montpellier vector platform | N/A |
AAV8-FLEX-scCas9 | Vector Biolabs | 7122 |
Ad-iN/WED | Leinninger, et al.22 | N/A |
AAV8-EF1α-DIO hChR2(H134R)-EYFP | UNC Gene Therapy Center | AV4378G |
AAV8-EF1α-DIO-eNpHR3.0-EYFP | UNC Gene Therapy Center | AV4846C |
AAV8-Abcc8/sgRNAs-FLEX-tdTOMATO | This paper | N/A |
AAV8-Ano4/sgRNAs-FLEX-tdTOMATO | This paper | N/A |
HSV-hEF1α-LS1L-GCaMP6f | MGH GDT Core | RN506 (HT) |
