Fig. 2: Survey of scRNA expression of cells from the aortic artery and the coronary artery.

a The transcriptomic profiling of aortic EC, SMC, and AF were compared with that of coronary arterial EC, SMC, and AF, respectively. Circos plot showing these site-specific genes were mainly shared by various cell populations from the same vascular bed. Lines, shared genes. b Violin plots of representative cell type specific markers. See Fig. 1 for cell-type abbreviations. c Immunofluorescence staining shows IL13RA2 (marker for AA_EC) expression in endothelial cells from the aortic artery. See also Supplementary Fig. 4b. d Immunofluorescence staining shows PTGS1 (marker for CA_EC1) expression in endothelial cells from the coronary artery. See also Supplementary Fig. 4c. e Immunofluorescence staining shows POSTN (marker for AA_SMC) expression in smooth muscle cells from the aortic artery. See also Supplementary Fig. 4d. f Immunofluorescence staining shows that Desmin and SORBS2 are markers for CA_SMC and both are expressed in smooth muscle cells from coronary arteries. See also Supplementary Fig. 5b and c. Scale bars (c–f), 50 μm. White squares (c–f) correspond to enlarged areas shown in the lower images. (c–f) Lu lumen; Ex extravascular space. g Networks of region-specific GO terms summarized by REVIGO. Differentially expressed genes (DEGs) between similar cell types (AA_EC vs. CA_EC1, AA_SMC vs. CA_SMC, AA_AF vs. CA_AF), were used to perform a GO analysis. Node colors indicate the –log₂ (p value); p values were corrected by the Benjamini-Hochberg algorithm; node size indicates the frequency with which the individual GO terms appeared. GO terms that are highly similar are linked by edges in the graph. h Schematic illustration of vascular organotypicity with region-specific markers and pathways.