Fig. 6: FGF1 mediates Piezo1-dependent regulation of adipocyte differentiation.
From: Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice
a Yoda1 (10 μM) effect on release of protein mediators (red) and lipids (black) from mouse adipocytes (ratio of release in the presence and absence of Yoda-1 (n = 5 mice)). b, c Effect of Yoda1 (10 µM) or hypotonic buffer (hypo; 210 mOsm/kg) on FGF1 release from vWAT adipocytes from wild-type (WT) and Ad-Piezo1-KO mice (KO) (b) or from human vWAT (c) (n = 3 mice (b), n = 4 samples (c)). d, e Effect of Yoda1 (10 μM; d) or hypotonic buffer (hypo; 210 mOsm/kg; e) on FGF1 release from mouse adipocytes pretreated in the absence or presence of brefeldin A (bref. A) or BAPTA-AM (n = 3 mice (control and bref. A in d as well as control and control + bref. A in e); n = 5 (control + hypo in e); n = 4 mice (BAPTA in d and all other groups)). f PD173074 (PD; 10 μM) effect on 3T3-F442A cell differentiation induced by conditioned medium of vWAT adipocytes from wild-type mice fed HFD for 16 weeks and effect of FGF1 (10 ng/ml) and FGF1 + PD173074 on differentiation of 3T3-F442A cells exposed to conditioned medium from vWAT adipocytes from KO animals. Control: non-conditioned medium. Cells were stained with Oil-red-O. g Differentiation of SVF cells from wild-type or Pdgfrα-CreERT2;Fgfr1flox/flox (Pα-Fgfr1-KO) mice exposed to conditioned medium from wild-type adipocytes from HFD-fed mice (Oil-red-O staining; n = 4 mice per condition). h H&E-stained vWAT sections from wild-type (WT) and Pα-Fgfr1-KO mice fed a HFD for 12 weeks (one representative of three independent experiments). i, j Average diameter (i) and total number (j) of adipocytes in vWAT from wild-type (WT) and Pα-Fgfr1-KO mice fed a HFD (n = 5 mice each). k Wild-type (WT) and Pα-Fgfr1-KO mice received BrdU during the first week of an 8-week HFD feeding period. Representative images and quantifications of immunofluorescence staining for BrdU in adipocyte nuclei of vWAT after HFD feeding (n = 5 mice (≥15 sections per mouse)). Bar lengths in f, g, h, k: 50 μm. Shown are mean values ± s.e.m.; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; n.s., not significant (two-tailed non-parametric Mann–Whitney U-test). Source data are provided as a Source data file.
